Ameneh Tarakemeh - Department of Horticultural Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Majid Azizi - Department of Horticultural Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Vahid Rowshan - Department of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.
Hassan Salehi - Department of Horticultural Science, Shiraz University, Shiraz, Iran.

Narcissus spp. are of great importance to pharmaceutical industries because of biosynthesis of Amaryllidaceae alkaloids. N. tazetta with several varieties is a widespread species, native to the Mediterranean region with considerable distribution in Iran. In the present study, calli, roots and bulblets were regenerated from N. tazetta var. Meskin. In order to report alkaloid profile and quantify galanthamine and lycorine, methanolic extract of different in vitro tissues were subjected to alkaloid purification by SPE (solid phase extraction) method, followed by GC-MS analysis. In general, galanthamine and lycorine were detected in all in vitro tissues, while demethylmaritidine was only identified in bulblets. Bulblet was identified as the best source of main Amaryllidaceae alkaloids regarding the highest quantities of galanthamine and lycorine with 15 and 731 µg/g dry weight (DW) respectively.

Amaryllidaceae alkaloids, Galanthamine, Lycorine, Narcissus, Tissue culture