Saber Ghaderpour - Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Rafighe Ghiasi - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Gholamreza Hamidian - Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Hamed Heydari - Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Rana Keyhanmanesh - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Objective(s): This research was designed to demonstrate the impact of voluntary exercise on sperm parameters including sperm count, morphology, motility, viability, testicular apoptosis, oxidative stress, and the mir-34a/SIRT1/p53 pathway in type 2 diabetic rats.Materials and Methods: 32 Wistar male rats were separated into four groups: control (C), voluntary exercise (VE), diabetic (D), and diabetic rats that performed voluntary exercise (VED). To induce diabetes, animals were injected with streptozotocin (35 mg/kg) after receiving a high-fat diet. The testicular protein levels of SIRT1 and P53, miR-34a expression, MDA, GPx, SOD, catalase, and sperm parameters were evaluated.Results: Diabetes caused increased testicular MDA content, miR-34a expression, acetylated p53 protein expression, and the percent of immotile sperm (p Conclusion: It seems that voluntary exercise has significant positive impacts that can be employed to reduce the complications of type 2 diabetes in the testis of male rats.

Apoptosis miR, 34a Oxidative stress p53 Sperm parameters Type 2 diabetes Voluntary exercise