Sara Bahonar - Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. & Molecular and cell biology Research center, Faculty of Medicine, Mazandaran University of Medical sciences, Sari, Iran.
Maryam Ghazvinian - Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science.
Mohamad Reza Haghshenas - Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science.
Hamid Reza Goli - Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science.
Bahman Mirzaei - Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Science. & Molecular and cell biology Research center, Faculty of Medicine, Mazandaran University of Medical sciences, Sari, Iran.

Background: Staphylococcus aureus is predominant at sites of biomaterial-associated infection (BAI) and frequently infects hospitalized individuals. Methods: The polysaccharide intercellular adhesin (PIA) and S. epidermidis rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host. Results: Purification of the macromolecules was confirmed by FTIR and Western blotting. Conclusions: The S. epidermidis SesC protein and PIA were successfully purified. Both are considered as vaccine candidates.

Biofilm formation, Polysaccharide Intracellular Adhesin, Staphylococcus aureus.