Fatemeh Bagherzadeh - Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Flora Forouzesh - Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Elham Rajabbeighi - Department of Developmental Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences,Islamic Azad University, Tehran, Iran.

Background and Aim: It has been reported that different species of Euphorbia have antitumor activity. Euphorbia Tehranica is considered a traditional herb which is distributed in Iran. The anticancer activity of ethyl acetate extract (EAE) from this herb against colorectal cancer is unclear. In this study, we evaluated the growth inhibitory effects of Euphorbia Tehranica extracts on human colon cancer cell line SW۴۸۰.Methods: The ethyl acetate was extracted from Euphorbia Tehranica. The SW۴۸۰ cells were treated with different concentrations of EAE (۵ to ۲۵۰ μg/ml) at ۲۴, ۴۸ and ۷۲ hours. Cell survival was evaluated using MTT assay. Anticancer activity of the EAE was assessed by evaluating the cell viability. Cell viability was determined at the wavelength of ۵۴۶ and ۶۳۰ nm by MTT method. The IC۵۰ was determined graphically.Results: In this regard, the EAE was found to markedly inhibit the proliferation of SW۴۸۰ cells in a dose and time-dependent manner. The cell mortality increased when the concentration of EAE increase from ۵µg/ml to ۱۰۰µg/ml. EAE treatment arrested growth in IC۵۰ concentration range. IC۵۰ for sw۴۸۰ cells was in ۵۰ µg/ml concentration after ۲۴ and ۴۸ hours and ۴۰ µg/ml after ۷۲ hours.Conclusion: We inferred that the EAE of Euphorbia Tehranica could have chemo preventive potential against human colorectal cancer. Our results may suggest that the gene expression which is contributed in apoptosis has been changed under pressure of EAE.

Euphorbia Tehranica, ethyl acetate, SW۴۸۰ cell line, cytotoxicity