Maryam Saedi - MSc Student in Cell and Molecular Biology, Kavian Institute of Higher Education
Nasser Mahdavi Shahri - Professor of Biology Ferdowsi University of Mashhad and Development and biotechnology Center-(Baft fanavaran tegarat pars)
saeedeh Samareh Mousavi - Assistant Professor of Biology, Kavian Institute of Higher Education
Amin Tavassoli - PhD in Biotechnology, Ferdowsi University of Mashhad and Development and Technology Center-(Baft fanavaran tegarat pars)

Background and Aim: Cellular and molecular sciences cover a wide range of academic disciplines, and It seems in the last few years with the development and application of new technologies in the celluhar and molecular sciences, the necessary background for the creation of a new trend, Matrix biology, has been provided. Technology and commercialization in the realm of this new trend can play a key role in the wealth creation process and in the knowledge economy. Applying specialist forces to university graduates, knowledge of new research in the field of biological matrix, Extraction and Identification of new Cyto-molecules and Biomolecules, Extraction of such biomolecules including Glycoconjugates Preparation of biofilms and diverse bioscaffold and biodressing are examples of new technology in the field of matrix biology and ECM derivative which along with the development of applied research and commercialization of the above products, will provide the fields of biobusiness in Iran, which with the development of applied research and the commercialization of the above products will provide the fields of biobusiness in Iran. In this article, we will present examples of such as products based on technologies related to identification, extraction and laboratory application. Then, we'll discuss the commercialization challenges of this products natural scaffold, especially in the ۳D model. (۳D) To perform tissue engineering research, the preparation and production of such a scaffold was the main objective of this research.Methods: For this study, ۱۰ ۲ to ۴ mm neck pieces from ۴ ۴-day-old mice were prepared as controls. The specimens were evaluated by hematoxylin eosin staining after fixation, dehydration, cryopreservation, paraffin staining and block preparation. The same number of neck fragments were extracted by Snap freezing method using SDS. After staining by hematoxylin eosin, blue toluidine and Masson's trichrome blue and AlCian blue and DAPI staining were studied.Results: This study showed that extracellular matrix structures in these scaffolds are well preserved after desiccation of mouse cervical fragments, despite removal of tissue cells. Technique scaffolds from the neck parts of the Biosscafold Mice provide suitable ۳D interactions for cell interactions.Conclusion: Freezing scaffolding provided in this research and access to a suitable freezing and freezing agenda to commercialize this three-dimensional model of natural scaffolding and conducting tests related to evaluating matrix cell interactions are future research ideas in this research. .

Biofilm - Tissue Model - Scaffolding Model - Scaffold Freezing – Degradation bioscaffold