Mustafa GULER - Department of Physiology, Atatürk University, Faculty of Medicine, Erzurum, Turkey
Ayhan Tanyeli - Department of Physiology, Atatürk University, Faculty of Medicine, Erzurum, Turkey
Derya GUZEL ERDOGAN - Department of Physiology, Sakarya University, Faculty of Medicine, Sakarya, Turkey
Ersen Eraslan - Department of Physiology, Yozgat Bozok University, Faculty of Medicine, Yozgat, Turkey
Selim Comakli - Department of Pathology, Atatürk University, Veterinary Faculty, Erzurum, Turkey
Elif Polat - Department of Nutrition and Dietetics, Faculty of Health Sciences, Erzurum Technical University, Erzurum, Turkey
Songul DOGANAY - Department of Physiology, Sakarya University, Faculty of Medicine, Sakarya, Turkey

Objective(s): This study aimed to determine anti-inflammatory, antioxidant, and antiapoptotic properties of urapidil (Ura) against ovarian torsion detorsion (T/D) injury in rats. Materials and Methods: ۴۰ female Wistar albino rats were grouped as sham, T/D, T/D+dimethyl sulfoxide (DMSO), T/D+Urapidil (Ura) ۰.۵ mg/kg (low dose), and T/D+Urapidil (Ura) ۵ mg/kg (high dose) groups. In treatment groups, Ura was administered intraperitoneally just before detorsion. Biochemical parameters (TAS, TOS, MDA, MPO, and SOD) and immunohistochemical (IL-۱β, TNF-α, NF-κB, LC۳B, and Caspase-۳) analyzes were performed.Results: In the T/D group, OSI and MPO levels were elevated significantly while TAS values decreased compared with the sham group. A significant difference occurred in the low dose treatment group in TAS and OSI levels compared with the T/D group. In the high dose treatment group, significant elevation in TAS but  reduction in OSI and MDA levels were observed compared with the T/D group. Immunohistochemical staining resulted in IL-۱β, TNF-α, NF-κB, LC۳B, and caspase-۳ immunopositivity in the T/D group, while Ura treatment decreased those parameters. Intensive congestion and hemorrhage were observed in the T/D group, but contrary to this, treatment groups had alleviated congestion and hemorrhage.Conclusion: These results suggest that Ura demonstrated protective effects against ovarian T/D injury via anti-oxidative, anti-inflammatory, and anti-apoptotic features. 

Autophagy, Caspase-۳, Detorsion, NF-κB, Ovarian, Torsion, Urapidil