Nakisa Zarrabi Ahrabi - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Yasin Sarve Ahrabi - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

Nowadays, drug resistance has become a serious issue in the treatment of cancers. ۱, ۳, ۴-Oxadiazole is a compound containing one oxygen atom and two nitrogen atoms in a five-membered ring, which is a major component of the development of new alternative therapies among oxadiazoles compounds. Therefore, the aim of this study was to investigate the antioxidant and anti-cancer properties of two methotrexate derivatives of ۱, ۳, ۴-oxadiazole against MCF-۷ breast cancer cells. In this study, new derivatives were first synthesized and then purified from the reaction between N-iso-cyanoimino triphenylphosphoran and carboxylic acid derivatives and then ۲-pyridine carbaldehyde in acetonitrile solvent. Antioxidant effects of the product were evaluated using ABTS and DPPH assays. The cytotoxicity effects of the compounds were evaluated by MTT assay towards breast cancer cells. The obtained results showed that the synthesized compounds had a significant effect on scavenging free radicals. Also, these compounds had cytotoxicity property towards MCF-۷ cells and reduced the survival of cancer cells in a dose-dependent manner. Based on our results, new derivatives of oxadiazoles that have methoxyphenyl in their original structure, have cytotoxicity as well as antioxidant properties towards MCF-۷ cancer cells and can be proposed as a suitable Structure in the field of pharmacy and medicine.

Drug resistance, oxadiazoles, free radicals, breast cancer cells