Masoume Vakili Azghandi - Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Mohammadreza Nasiri - Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Ali Shamsa - Department of Urology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohsen Jalali - Department of Urology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Mahdi Shariati - Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Background: The SRY gene (SRY) provides instructions for making a transcription factor called the sex-determining region Y protein. The sex-determining region Y protein causes a fetus to develop as a male. In this study, SRY of ۱۵ spices included of human, chimpanzee, dog, pig, rat, cattle, buffalo, goat, sheep, horse, zebra, frog, urial, dolphin and killer whale were used for determine of bioinformatic differences. Methods: Nucleotide sequences of SRY were retrieved from the NCBI databank. Bioinformatic analysis of SRY is done by CLC Main Workbench version ۵.۵ and ClustalW (http:/www.ebi.ac.uk/clustalw/) and MEGA۶ softwares. Results: The multiple sequence alignment results indicated that SRY protein sequences from Orcinus orca (killer whale) and Tursiopsaduncus (dolphin) have least genetic distance of ۰.۳۳ in these ۱۵ species and are ۹۹.۶۷% identical at the amino acid level. Homosapiens and Pantroglodytes (chimpanzee) have the next lowest genetic distance of ۱.۳۵ and are ۹۸.۶۵% identical at the amino acid level. Conclusion: These findings indicate that the SRY proteins are conserved in the ۱۵ species, and their evolutionary relationships are similar.

Bioinformatics analysis, SRY gene, Phylogeny