Identification and Molecular Characterization of the cDNA Encoding Cucumis melo Allergen, Cuc m ۳, a Plant Pathogenesis-Related Protein
عنوان مقاله: Identification and Molecular Characterization of the cDNA Encoding Cucumis melo Allergen, Cuc m ۳, a Plant Pathogenesis-Related Protein
شناسه ملی مقاله: JR_RBMB-2-2_004
منتشر شده در در سال 1393
شناسه ملی مقاله: JR_RBMB-2-2_004
منتشر شده در در سال 1393
مشخصات نویسندگان مقاله:
Mojtaba Sankian - Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Jafar Hajavi - Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Malihe Moghadam - Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Abdol-Reza Varasteh - Allergy Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
خلاصه مقاله:
Mojtaba Sankian - Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Jafar Hajavi - Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Malihe Moghadam - Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Abdol-Reza Varasteh - Allergy Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Background: Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m ۱ and Cuc m ۲, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m ۳.
Methods:The Cuc m ۳ cDNA has been characterized by rapid amplification of cDNA ends (RACE), which revealed a ۴۵۶ base-pair (bp) fragment encoding a ۱۵۱-amino acid polypeptide with a predicted molecular mass of ۱۶.۹۷ kDa, and identified ۷۹ and ۱۷۸ bp untranslated sequences at the ۵′ and ۳´ ends, respectively.
Results: In silico analysis showed strong similarities between Cuc m ۳ and other plant pathogen-related protein ۱s from cucumber, grape, bell pepper, and tomato.
Conclusion: Here we report the identification and characterization of the Cuc m ۳ cDNA, which will be utilized for further analyses of structural and allergenic features of this allergen.
کلمات کلیدی: Allergen, Cuc m ۳, Melon, Plant pathogenesis-related protein ۱
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1263153/