Baohua Su - Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰
Deng Li - Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰
Jie Xu - Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰
Yingbin Zhang - Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰
Zhiqing Cai - Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰
Max Kauther - Department of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Hufelandstrasse ۵۵, ۴۵۱۴۷ Essen, Germany
Ruofan Ma - Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, ۵۱۰۱۲۰

Objective(s): The study aimed to uncover the underlying mechanism linking wear particles to osteoclast differentiation, and we explored the effect of titanium particles of different sizes on CD۱۴۷ expression and autophagy in macrophages. Materials and Methods: Effects of titanium particles on CD۱۴۷ and RANKL mRNA were detected by QPCR; protein level of CD۱۴۷ and Beclin-۱ were detected by Western blot; soluble RANKL were detected by ELISA. To determine the effect of CD۱۴۷ and autophagy, KG-۱a cells were transfected with siRNA-CD۱۴۷ or treated with autophagy inhibitor CQ (chloroquine), and then co-cultured with different sizes of titanium particles.Results: Our results showed that ۰.۲-۱.۲ µm and ۱.۲-۱۰ µm titanium particles up-regulate CD۱۴۷ to activate autophagy, which increase the level of soluble RANKL to promote osteoclastogenesis. Suppression of CD۱۴۷ with siRNA could diminish particle-induced autophagy and soluble RANKL expression. In addition, CQ could dramatically reduce particle-induced soluble RANKL expression. Conclusion: Our findings suggested a possible mechanism underlying wear debris-induced osteolysis and identified CD۱۴۷ as a potential therapeutic target in aseptic loosening.

Autophagy, CD۱۴۷, Osteoclastogenesis, Peri-implant osteolysis, RANKL