Zahra Boghrati - Department of Pharmcognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Maryam Naseri - Department of Pharmcognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Mitra Rezaie - Department of Nutrition, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Ngoc Pham - Eskitis Institute, Griffith University, Brisbane, QLD ۴۱۱۱, Australia
Ronald J Quinn - Eskitis Institute, Griffith University, Brisbane, QLD ۴۱۱۱, Australia
Zahra Tayarani-Najaran - Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Mehrdad Iranshahi - Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Objective(s): In food industry, the inhibition of tyrosinase is very important, because this enzyme catalyzes the oxidation of phenolic compounds found in fruits and vegetables into quinones, which contribute in undesirable color and taste of fruits and vegetables. Teucrium polium L. var. gnaphalodes (Lamiaceae), a wild-growing flowering plant that has many applications in food preparations and traditional medicine. In Persian language, this medicinal herb is called Kalpoureh. Materials and Methods: ۱D- and ۲D-NMR experiments were used to determine the chemical structures of the isolated compounds. Antioxidant and tyrosinase inhibitory activities of the isolated compounds were evaluated using DPPH, FRAP and mushroom tyrosinase inhibition assays. Results: In this research, we isolated two phenylpropanoid glycosides including verbascoside and poliumoside and two flavonoids including jaranol and isorhoifolin using chromatographic techniques. We found promising antioxidant and anti-tyrosinase compounds from Teucrium polium L. var. gnaphalodes. Conclusion: To date, different compounds have been isolated and characterized from T. polium including terpenoids and flavonoids. But no phytochemical study has been reported from T. polium var. gnaphalodes. Poliumoside and jaranol showed promising antioxidant and tyrosinase inhibitory activities, respectively.

DPPH, FRAP, Isorhoifolin, Jaranol, Lamiaceae, Mushroom tyrosinase - inhibition assay, Poliumoside, Teucrium polium var.- gnaphalodes