Detection of TEM, SHV and PER Type Extended-Spectrum ß-Lactamase Genes among Clinical Strains of Pseudomonas aeruginosa Isolated from Burnt Patients at Shafa-Hospital, Kerman, Iran
عنوان مقاله: Detection of TEM, SHV and PER Type Extended-Spectrum ß-Lactamase Genes among Clinical Strains of Pseudomonas aeruginosa Isolated from Burnt Patients at Shafa-Hospital, Kerman, Iran
شناسه ملی مقاله: JR_IJBMS-11-2_008
منتشر شده در در سال 1387
شناسه ملی مقاله: JR_IJBMS-11-2_008
منتشر شده در در سال 1387
مشخصات نویسندگان مقاله:
Mohammad Reza Shakibaie - Department of Mirobiology, Kerman University of Medical Sciences, Kerman, Iran
Fereshteh Shahcheraghi - Department of Bacteriology, Institute Pasteur of Iran, Tehran, Iran
Ali Hashemi - Department of Mirobiology, Kerman University of Medical Sciences, Kerman, Iran
N Saeed Adeli - Department of Mirobiology, Kerman University of Medical Sciences, Kerman, Iran
خلاصه مقاله:
Mohammad Reza Shakibaie - Department of Mirobiology, Kerman University of Medical Sciences, Kerman, Iran
Fereshteh Shahcheraghi - Department of Bacteriology, Institute Pasteur of Iran, Tehran, Iran
Ali Hashemi - Department of Mirobiology, Kerman University of Medical Sciences, Kerman, Iran
N Saeed Adeli - Department of Mirobiology, Kerman University of Medical Sciences, Kerman, Iran
Objective(s) This study was carried out to evaluate the existence of the TEM, SHV and PER ESBL genes in ESBL producing strains of Pseudomonas aeruginosa isolated from burnt patients at Shafa-hospital, Kerman, Iran. Materials and Methods A total of ۱۲۰ strains of P. aeruginosa were isolated from ۲۴۵ patients in burn unit of Shafa-hospital during January ۲۰۰۶ to December ۲۰۰۷. MIC of antibiotics was measured using agar dilution test. ESBL producing strains were detected by double-disc synergy method containing amoxicillin and amoxicillin+clavulanic acid and phenotypic confirmatory test. All the clinical isolates resistant to imipenem (IMP) were screened for the production of MBL by E-test with IMP/IMP+EDTA strips. PCR and multiplex-PCR performed for the detection of different types of ESBL producing genes in ESBL positive isolates. Results Of ۱۲۰ the isolates, ۳-۵% showed MIC greater than ۱۶ µg/ml to IMP and meropenem, ۶۶% showed MIC greater than ۳۲ µg/ml to ceftazidime, ۴۲% to azteronam and ۶۰% of the isolates showed MIC greater than ۶۴ µg/ml to cefotaxime, ۴۱ (۳۴%) confirmed as ESBL producers. Not any isolate could produce MBL (P≤۰.۰۵). The PCR assay of all ESBL producing isolates revealed that ۶.۶%, ۴.۱% and ۲.۵% of them were positive for SHV, PER and TEM genes, respectively. Conclusion Many ESBL producing strains of P. aeruginosa isolated from patients in burn unit of Shafa-hospital. However, none could produce MBL enzyme. The genes among ESBL producing strains were SHV, PER as well as TEM type of β-lacatamases.
کلمات کلیدی: Antibiotic resistance, ESBL, MBL, Pseudomonas aeruginosa
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1296799/