Li-qun Xiao - Department of Stomatology, The First College of Clinical Medical Science of China Three Gorges University, Yichang Central People&#۰۳۹;s Hospital, Yichang ۴۴۳۰۰۳, China
Hong-tao Wang - Department of Stomatology, The First College of Clinical Medical Science of China Three Gorges University, Yichang Central People&#۰۳۹;s Hospital, Yichang ۴۴۳۰۰۳, China
Yu-lan Li - Department of Stomatology, The First College of Clinical Medical Science of China Three Gorges University, Yichang Central People&#۰۳۹;s Hospital, Yichang ۴۴۳۰۰۳, China
Qing Zeng - Department of Stomatology, The First College of Clinical Medical Science of China Three Gorges University, Yichang Central People&#۰۳۹;s Hospital, Yichang ۴۴۳۰۰۳, China
E Zhou - Department of Stomatology, The First College of Clinical Medical Science of China Three Gorges University, Yichang Central People&#۰۳۹;s Hospital, Yichang ۴۴۳۰۰۳, China
Xia Ni - Department of Stomatology, The First College of Clinical Medical Science of China Three Gorges University, Yichang Central People&#۰۳۹;s Hospital, Yichang ۴۴۳۰۰۳, China
Zhong-ping Huan - Department of Stomatology, The First College of Clinical Medical Science of China Three Gorges University, Yichang Central People&#۰۳۹;s Hospital, Yichang ۴۴۳۰۰۳, China

Objective(s): Salvia miltiorrhiza (SM) is a popular and classic herb in traditional Chineses medicines. The objective is to confirm the effects of aqueous extract of S. miltiorrhiza (ESM) and its main ingredient on the promotion of orthodontic tooth movement and healing of periodontal ligament in rat. Materials and Methods: Male Sprague-Dawley rats (n= ۱۵۰) were divided into five groups: model control group (۰.۵ ml/kg phosphate-buffered saline (PBS) injection), ESM group (۰.۷۵ g/kg/day of crude drugs) and Danshensu subgroups (۲۵۰, ۵۰۰, ۷۵۰ mg/kg/day of body weight). All rats were administered intramuscularly into the buccal vestibular mucosa of first molar of left maxillary. The indicators such as the moving distance of orthodontic tooth, nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) expression and osteoclasts were tested. Results: The expressions of RANKL and OPG in the treatment groups were obviously enhanced compared with control group (P<۰.۰۵). The increase rate of OPG expression was slower than that of RANKL. But, RANKL decreased conspicuously after no orthodontic pressure was applied, especially in the treatment groups (Danshengsu high dose group at day ۳۰: ۲.۱۷ versus ۳.۴۷ of control, P<۰.۰۱). ESM groups promoted osteoclasts proliferation in the first ۲۰ days. Conclusion: There is a relationship between RANKL/OPG ratio and the number of osteoclasts. ESM might accelerate periodontal alteration of rat orthodontic tooth via producing more osteoclasts.

Danshensu, Movement distance, Osteoclasts, Osteoprotegerin, Receptor activator for nuclear factor κB ligand