Jamshid Faghri - Department of Bacteriology and Virology, Faculty of Medicine , Isfahan University of Medical Sciences, Isfahan, Iran
Delavar Shahbazzadeh - Biotechnology Research Centre, Pasteur Institute of Iran, Tehran, Iran
Kamran Pooshang Bagheri - Biotechnology Research Centre, Pasteur Institute of Iran, Tehran, Iran
Sharareh Moghim - Department of Bacteriology and Virology, Faculty of Medicine , Isfahan University of Medical Sciences, Isfahan, Iran
Hajieh Ghasemian Safaei - Department of Bacteriology and Virology, Faculty of Medicine , Isfahan University of Medical Sciences, Isfahan, Iran
Bahram Nasr Esfahani - Department of Bacteriology and Virology, Faculty of Medicine , Isfahan University of Medical Sciences, Isfahan, Iran
Hossein Fazeli - Department of Bacteriology and Virology, Faculty of Medicine , Isfahan University of Medical Sciences, Isfahan, Iran
Rahmatolah Yazdani - Department of Bacteriology and Virology, Faculty of Medicine , Isfahan University of Medical Sciences, Isfahan, Iran
Hamid Mirmohammad Sadeghi - Department of Biotechnology, Faculty of pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Objective(s) Staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. Antibiotic therapy for vancomycin resistant S. aureus (VRSA) can not promise the eradication of infections. Since adhesion is the major route of infections, adhesin based vaccine could suppress S. aureus infections. Fibronectin binding protein A (FnBPA) and clumping factor A (ClfA) are major responsible adhesions involved in S. aureus infections, so they could be candidate vaccine molecules against an extensive range of infections. This project intended to express a new fusion protein construct and analysis of biological activity regarding binding activity. Materials and Methods pfnbA- ClfA construct was transformed to Escherichia coli BL۲۱ (DE۳). Transformant E. coli were grown in LB broth and induced with IPTG and cellular extracts were separated on SDS–PAGE. RT-PCR was performed to verify expression. Binding activity of fusion protein was studied using human gingival fibroblast (HGF) cell line. D۱-D۳ protein from unpublished study was used as control. Results The expected fusion protein fragment showed by SDS-PAGE. RT-PCR verified the existence of mRNA relating to expressed fusion protein. Binding activity of S. aureus decreased after treatment of HGF cells with fusion protein. Conclusion In total,binding activity of fusion protein was approximately two fold lesser than D۱-D۳ protein. It is supposed that the fusion protein could not be attached to its ligand easily and would be more accessible to antigen presenting cells and consequentlyprotective antibodies will be produced. This project is pending for in vivo infection study in animal model. 

Adhesion, Clumping factor A, Fibronectin binding protein A, Fusion protein, Staphylococcus aureus, Two dimensional structure