Ebrahim Asadi - Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
Mehrdad Jahanshahi - Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran
Mohammad Jafar Golalipour - Gorgan Congenital Malformation Research Centre, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iran

Objective(s) Cigarette and nicotine enhances embryogenesis, fertility, pregnancy loss and ultrastructure alterations of oocyte. This study was performed to determine the effect of daily supplementation of vitamin E on oocytes apoptosis in nicotine-treated mice. Materials and Methods In this experimental study, ۲۴ NMARI adult female mice were randomly allocated into four experimental groups. For ۳۰ days, animals in control group (C) were received saline through subcutaneous injection, group I received vitamin E (۶۰ mg/kg/day orally), group II received nicotine (۵ mg/kg/day, subcutaneous) and animals of group III received nicotine with vitamin E (۶۰ mg/kg/day orally). After ۳۰ days, the animals were superovulated with PSMG (۱۰ Units) and HCG (۱۰ Units). Next day animals were sacrificed and oocytes were flushed.  Collected oocytes were examined through TUNEL assay for the determination of apoptosis through the use of fluorescent microscope. Results The number of retrieved oocytes was ۱۳۹, ۱۴۸, ۹۷ and ۱۲۷ in control, experimental group I, II and III, respectively. Nicotine treatment increased apoptosis in oocytes up to ۱۳.۴% whereas oocytes apoptosis was ۳.۶% in controls. Supplementation with vitamin E in nicotine-treated mice reduced the oocytes apoptosis to ۵.۵%. Conclusion This study showed that nicotine exposure (۵ mg/kg/day for ۳۰ days) can increase apoptosis in oocytes, and supplementation with vitamin E (۶۰ mg/kg/day orally) can reduce the oocytes apoptosis in nicotine-treated mice.  

Antioxidant, Cell death, Nicotine, Oocytes, TUNEL assay