Leila Soltani - Department of Animal Sciences, Faculty of Agriculture and engineering, Razi University, Kermanshah, Iran
Maryam Darbemamieh - Department of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iran .

Ziziphus Jujube L. (Rosales: Ramnaceae) is a fruit tree that grows with different characteristics and flavors in some tropical and subtropical regions of the world. This fruit has been used as food and medicine. Phytochemical studies on Z. jujube have led to the extraction and identification of various alkaloids, flavonoids, tannins, sterols and saponin. The aim of this study was to evaluate the anti-proliferative properties of Z. Jujube aqueous extract in low concentrations on breast cancer cells (Mcf-۷). After preparation of Z. Jujube fruit, its kernels were separated and the fruit was dried. The concentrations of ۳۰, ۶۰, ۱۲۰ and ۲۴۰ μg/ml from this extract were added to the cell culture medium. The non-treated group was considered as a control. Anti-proliferative properties were evaluated by MTT assay. Data were analyzed in SPSS software and a significance level of ۵% was considered. Addition of ۲۴۰ μg/ml aqueous extract to the culture medium of cancer cells had the greatest toxicity effect compared to other concentrations of aqueous extract (p<۰.۰۵). In this study, the anti-proliferative effects of different concentrations were dose-dependent. Finally, it seems that Z. Jujube aqueous extract has relative anti-proliferative effects at high concentrations, which used in this essay.

Aqueous extract, Anti-cancer, Cytotoxicity, Jujube, Apoptosis