Mahdiyeh Ghafari - Assistant Professor in Exercise Physiology, Shahrekord University, Shahrekord, Iran
Ebrahim Banitalebi - Associate Professor in Exercise Physiology, Shahrekord University, Shahrekord, Iran
Mohamad Faramarzi - Associate Professor in Exercise Physiology, Shahrekord University, Shahrekord, Iran

Background and aims: Intermuscular lipolysis disorder plays an important role in insulin resistance and diabetes mellitus and perilipinPLIN۵ and PLIN۳ are the key proteins in regulating muscle cellular lipolysis. Therefore, the purpose of this study was to examine therelationship between the expression of PLIN۳ and PLIN۵ protein following endurance training in streptozotocin (STZ) rats.Methods: A number of ۲۴ male Wistar rats were randomly divided into low endurance training group (n = ۸), high-intensity training group(n = ۸), and control group (n = ۸). Diabetes was induced in every rat by STZ injection. Three days after injection, the blood samples weretaken from the cut tip of the tails of the mice and animals with blood glucose greater than ۳۰۰ mg/dL were considered diabetic. The trainingprogram included eight weeks of aerobic training at different intensities. Training in high- and low-intensity groups included ۲۲-۲۵ and ۵-۸m/min of training. Finally, one-way analysis of variance (ANOVA) and correlation was used to determine the significance of the differencesbetween variables, followed by utilizing Tukey’s post-hoc test for significance.Results: The comparison between the groups by ANOVA showed significant differences in PLIN۳ (P = ۰.۰۰۰۶) and PLIN۵ (P = ۰.۰۱۲).The results of Tukey post hoc test also demonstrated a statistical difference between the mean values of diabetic control group andhigh-intensity endurance group regarding PLIN۳ (P = ۰.۰۱) and PLIN۵ (P = ۰.۰۰۹), but no significant increase was observed in the lowintensityexercise group as compared to the control group (PLIN۳, P = ۰.۰۶۷ & PLIN۵, P = ۰.۴۴). As regards insulin resistance, there wasa significant difference among the three groups (P = ۰.۰۰۰۱). Eventually, the result of the correlation between PLIN۳ and PLIN۵ showedsimilar enhancement by increasing the intensity (P = ۰.۰۰۲۶).Conclusion: According to research results, high-intensity endurance training increased the expression of PLIN۳ and PLIN۵ in diabeticspecimens and PLIN۳ and PLIN۵ followed a similar increase pattern in high-intensity training.

Diabetes, PLIN۵, PLIN۳, Endurance Training, Intramuscular Triglycerides, Insulin Resistance