Devidas C. Pawar - School of Chemical science, Swami Ramanand Treeth Marathwada University, Nanded- ۴۳۱۶۰۶, India
Sunil V. Gaikwad - Department of Chemistry, Dr.D. Y. Patil ACS Womens College Pimpri, Pune-۴۱۱۰۱۸; Affiliated to Savitribai Phule Pune University, India
Sonali S. Kamble - Department of Biochemistry, Gramin Science (Vocational) College, Vishnupuri, Nanded-۴۳۱ ۶۰۶ (MS), India
Priya D. Gavhane - School of Chemical science, Swami Ramanand Treeth Marathwada University, Nanded- ۴۳۱۶۰۶, India
Milind V. Gaikwad - Department of Chemistry, Dr. D. Y. Patil ACS College Pimpri, Pune - ۴۱۱۰۱۸; Affiliated to Savitribai Phule Pune University, India
Bhaskar S. Dawane - School of Chemical science, Swami Ramanand Treeth Marathwada University, Nanded- ۴۳۱۶۰۶, India

A new series of (E)-۴-((۴-substitutedphenyl)diazenyl)-۱H-pyrazole-۳,۵-diamine derivatives were synthesized by using greener poly ethylene glycol-۴۰۰ as a reaction solvent. The drug resistance capacity and growing incidence of Mycobacterium tuberculosis infections have been increasing day by day. There is high demand for the synthesis of multidrug resistance anti-tubercular drugs. Herein, all the newly prepared ligand was subjected to the Vitro anti-tubercular activity against Mycobacterium tuberculosis against H۳۷Rv strains with, pyrazinamide, isoniazid, ethambutol, and streptomycin as the standard drugs. The compounds ۴a, ۴b and ۵a showed excellent activity while ۵b exhibit moderate activity against Mycobacterium tuberculosis. Further, the molecular docking was done with an enzyme PDB:۱G۳U. The ligand thymidine monophosphate was completed in the crystal structure of mycobacterium tuberculosis, while Mtb Pks۱۳ Thioesterase domain in complex with inhibitor TAM۱۶ with PDB:۵V۳Y Crystal Structure showed excellent docking results. These newly synthesized derivatives of pyrazole may be useful in the development of new anti-tubercular agents.

Mycobacterium Tuberculosis, Pyrazole derivatives, PEG-۴۰۰