In-silico study of rs۱۸۰۱۱۵۷ and hsa-mir-۱۴۹-۵p single nucleotide polymorphisms related to CLCX۱۲ gene in patients with leukemia

Backgrounds: Acute myeloid leukemia (AML) is a cancer of the myeloid line of blood cells,characterized by the rapid growth of abnormal cells that build up in the bone marrow and bloodand interfere with normal blood cell production. Chemokine-receptor axes are defined as factorsinvolved in AML pathogenesis and prognosis. The chemokine receptor CXCR۴ along with itsligand, CXCL۱۲ fit in important players that are actively involved in the cross-talk betweenleukemia cells and bone marrow microenvironment. The CXCL۱۲ or SDF۱ gene is anantimicrobial gene that encodes an alpha-derived stromal cell. CXCL۱۲ is produced by the BMmicroenvironment, binds and activates its cognate receptor CXCR۴ on leukemic cells, facilitatesleukemia cell trafficking and homing in the BM microenvironment, and keeps leukemic cells inclose contact with the stromal cells and extracellular matrix that constitutively generate growthpromotingand anti-apoptotic signals.Materials and Methods: A gene named is CXCL۱۲ one of the genes linked with this cancer.MicroRNAs linked to this gene were discovered using the miRNASNP database. A singlenucleotide polymorphism called rs۱۸۰۱۱۵۷ was discovered. The DAVID database of thismicroRNA picked the corresponding signals for further analysis.Results: For OC, ۱۲۰۰ genes have been discovered. CLCX۱۲ genes were shown to have a highlevel of expression, while hsa-mir-۱۴۹-۵p had up-expression examination of OC signalingpathways, as well as the influence of this miR on gene targets.Conclusion: These findings imply that CLCX۱۲ could be a potential gene therapy target forgastric cancer. Blocking CLCX۱۲ expression in leukemia cells induces apoptosis, which mayprovide a novel treatment approach.