Masoumeh Dolati - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
Farzaneh Tafvizi - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
Masoud Salehipour - Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran
Tahereh Komeili Movahed - Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
Parvaneh Jafari - Microbiology Department, Faculty of Science, Arak Branch, Islamic Azad University, Arak, Iran

Background and Aim: Breast cancer is one of the most common types of cancer among Iranian women. To date, the usual cancer treatments have not been entirely effective. Therefore, creating anticancer products is of great importance. The aim of this study was to evaluate the cytotoxic, anticancer, and induction effects of Bacillus coagulans probiotic bacterial supernatant on SKBR۳ cells. Materials and Methods: The anticancer potential and cytotoxic effect of different concentrations of probiotic bacterial supernatants (۱, ۲, ۳, ۴, ۵, ۶ and ۷ mg/mL) were evaluated on SKBR۳ cells for ۲۴, ۴۸, and ۷۲ h by MTT technique. QRT-RCR was used to assess the expression of bax, bcl۲, casp۳, and casp۹ genes, and flow cytometry was used to evaluate apoptosis in cancer cells. Results: The inhibitory effect of dose- and time-dependent B. coagulans supernatant showed that the supernatant of this probiotic bacterium had a cytotoxic effect on SKBR۳ cancer cells. On the other hand, analysis of flow cytometry results and increased expression of bax, casp۳, and casp۹ pro-apoptotic genes and decreased bcl۲ expression in cancer cells showed induction of apoptosis. Conclusion: The anticancer and cytotoxic effect of B. coagulans probiotic bacterial supernatant on SKBR۳ cancer cells shows that with further research, this probiotic bacterium can be used as a new strategy for the possible treatment of breast cancer.

Apoptosis, Bacillus coagulation, Breast cancer, Probiotic, SKBR۳ cancer cells, Supernatant, آپوپتوز, باسیلوس کوآگولانس, پروبیوتیک, سرطان پستان, سلول SKBR۳, سوپرناتانت