علی منصوری - Department of Agriculture, Faculty of Agriculture, Shahed University, Tehran
حشمت امیدی - Department of Agriculture, Faculty of Agriculture, Shahed University, Tehran

To investigate the effect of quinine seed priming on morpho-physiological and biochemical traits of seedlings, a factorial experiment was conducted in a completely randomized design with four replications at the seed processing laboratory of the Shahed University of Tehran. Experimental factors included two levels of seed age (one-year old and four-year old) and four priming treatments (control without priming, hydro-priming, salicylic acid with a concentration of ۵۰ ppm and potassium nitrate with a concentration of ۰.۲ wt% by volume). The effect of seed age on all studied traits was significant at the level of ۱% probability. With increasing seed age, germination percentage (۴۵.۶%), germination rate (۶۳.۴۳%), seedling length (۵۹.۲۱%), chlorophyll a content (۵۳.۱%), chlorophyll b content (۶۷.۴%), carotenoids (۶ ۵۶.۶%), soluble sugar (۵۵.۸%), protein (۴۷%), anthocyanin (۶۲%) and catalase activity (۵۰%) decreased whereas the amount of electrolyte leakage increased by ۲۵.۹%. The effect of priming on all studied traits was significant at the level of one percent probability and improved the studied traits. The interaction effect of seed age and priming on some traits was also significant. Priming of four-year-old seeds with potassium nitrate as the best treatment composition increased Germination percentage (۵۲.۶۷%), Germination rate (about ۴۹۰%), Shoot length (about ۴۳۰%), Chlorophyll a content (۷۷.۴%), Chlorophyll b content (۱۸۰%), Carotenoids (۹۲.۸%), Protein (۲۰۰%) and Catalase activity (۲۵۰%) and ۴۰.۲% reduction of Electrolyte leakage from the plasma membrane. According to the obtained results, it can be stated that the use of the potassium nitrate priming method can greatly reduce the effects of ageing and seed deterioration.  

Priming, protein, seed aging, catalase, potassium nitrate, پرایمینگ, پروتئین, پیری بذر, کاتالاز, نیترات پتاسیم