Fujiati Fujiati - Department of Biochemistry and Biomolecular, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia.
Haryati Haryati - Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia.
Joharman Joharman - Department of Pharmacology and Therapy, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia.
Sabrina Wahda Utami - Undergraduate Medical Education, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia.

Background: Rhodomyrtus Tomentosa (Karamunting) is one of South Kalimantan's biodiversity. In previous research on asthma and coal dust exposure models, nebulization with an ethanol extract of R. tomentosa leaves has been shown to reduce inflammatory cells. This study aimed to investigate the anti-inflammatory activity on the stabilization of red blood cell membranes and to identify the chemical compounds present in extracts of R. tomentosa leaves.   Methods: Anti-inflammatory effect of R. tomentosa leaves was evaluated by in vitro red blood cell membrane stabilization methods. Nonsteroidal anti-inflammatory sodium diclofenac was used as the reference drug. The content of chemical compounds in the ethanol extract of R. tomentosa leaves was identified using Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). Results: The results of inhibition of red blood cells membrane lysis showed the n-hexane fraction (concentration ۲۵ ppm), ethyl acetate fraction (concentration ۵۰ ppm), and a fraction of water (concentration ۵۰ ppm) with an inhibition level of ۵۴.۵%, ۸۱.۸%, ۶۳.۶%, respectively. The ethyl acetate fraction showed the highest inhibition of hemolysis. This result is not significantly different from the standard anti-inflammatory sodium diclofenac (۹۰.۰۹%). Oleanonic acid and ursonic acid were two similar metabolites in subfractions ethyl acetate ۱, ۲, and ۳, which may have anti- inflammatory properties. Conclusions: R. Tomentosa leaves can have potency as an anti-inflammatory by increasing the red blood cell membrane stability equal to lysosome cells, depending on the concentration.

Anti-inflammatory, Oleanonic acid, Ursonic acid, Rhodomyrtus tomentosa