سمیه احمدی - Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of medical Sciences, Yazd, Iran
گیلدا اسلامی - Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of medical Sciences, Yazd, Iran
محمود وکیلی - Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of medical Sciences, Yazd, Iran
کاظم برزگر - Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of medical Sciences, Yazd, Iran
علی فتاحی بافقی - Department of Medical Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Infectious Diseases Research Center, Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Background and Aims: Cutaneous Leishmaniasis is a significant health problem in many parts of Iran. Management of the disease and its treatment is a global dilemma. In this study, the status and the proportions of Cutaneous Leishmaniasis induced by Leishmania major and tropica among suspected patients referred to the Health Centers of Abarkouh, Ardakan, Bafgh, and Khatam cities, Yazd Province, Iran were investigated. Materials and Methods: The lesion was diagnosed using direct smear microscopy and conventional polymerase chain reaction. Results: A total of ۹۰ samples were prepared of which ۶۴ (۷۱.۱%)  were male and ۲۶ (۲۷.۹%) were female. Also, ۳۰ (۳۳.۳%) samples came  from Ardakan, ۲۹ (۳۲.۲%) samples  from Bafgh, ۲۱ (۲۳.۳%) samples from Abarkouh, and ۱۰ (۱۱.۱%) samples  from Khatam city. Ninety samples with ۱۱۲ lesions were recruited and parasitologically examined. The results showed that, in macroscopic examination, ۹۰ of the patients had ۵۹ lesions: patients with lesion(s) induced by Leishmania major = ۵۲ (M=۳۵, ۶۷.۳%, F=۱۷, ۳۲.۷%), lesion(s) induced by Leishmania tropica =۴ (M=۲, ۵۰.۰%, F=۲, ۵۰.۰%), and lesion(s) induced by false positive =۳۳ (۳۶.۴%). Basides, in microscopic method, ۹۰ of the patients had ۵۹ lesions: patients with lesion(s) induced by Leishmania major =۴۴ (M=۲۹, ۶۵.۹%, F=۱۵, ۳۴.۰۹%), lesion(s) induced by Leishmania tropica =۹ (M=۸, ۸۸.۸%, F=۱, ۱۱.۲%), and lesion(s) induced by false positive =۳۷ (M=۲۸, ۷۵.۷%, F=۹, ۲۴.۳%).  Conclusion: Although travel history to an endemic area is important for diagnosis, parasitological confirmation is necessary to initiate treatment.

Cutaneous Leishmaniasis, Leishmania major, Leishmania tropica, PCR, Yazd, سالک, ابرکوه, اردکان, بافق, خاتم, PCR