الهه پیرزاده - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
سیدحمید آقایی بختیاری - Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
ندا یعقوبی - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
علی محمودی - Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
لیدا جراحی - Department of Community Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
عباس عبداللهی - Department of Surgury, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
سداسحاق هاشمی - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
سیدمهدی حسنیان - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
فرناز زاهدی اول - Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Background and Aims: Colorectal cancer (CRC) is one of the most common human cancers. Currently, carcinoembryonic antigen (CEA) is used as the main standard biomarker of CRC, though this biomarker is not specifically made for CRC and, in a minority of cases, shows inadequate sensitivity. Therefore, searching for novel accessory biomarkers may fill these gaps in clinical management. miRNAs physiologically regulate various metabolic processes and are misregulated in various cancers. Therefore, the present investigation was conducted to evaluate miR-۱ levels in CRC samples. Materials and Methods: The CRC and adjacent tissue samples were obtained from ۲۴ patients. In addition, sera were collected from the patient group and ۲۴ healthy controls. Total RNA was extracted from tissue samples, and cDNA was synthesized. Real-time PCR determined the expression of miR-۱. Serum levels of CEA were also measured using a Monobind ELISA assay kit. Results: The level of miR-۱ in CRC tumors was significantly down-regulated. Moreover, patients with metastasis showed lower expression of miR-۱ compared to cases without metastasis; however, this difference was not statistically significant. The ROC curve for miR-۱ showed an AUC of ۰.۶۹. In addition, ROC analysis revealed a sensitivity of ۷۰.۲۷% and a specificity of ۶۲.۹۶% for miR-۱. Conclusion: There is still a need for new upcoming markers in addition to the main CRC biomarker, CEA. The levels of miR-۱ in colorectal cancer tissue samples may provide additional information for the management and follow-up of CRC patients; though, the clinical application needs further studies.

Carcinoembryonic antigen, Colorectal cancer, miR-۱, Tumor biomarker, نشانگر زیستی, آنتی ژن کارسینوامبریونیک, کانسر کولورکتال, mir-۱