R Sedghi - Research Laboratory of Spectrometry & Micro and Nano Extraction, Department ofChemistry, Iran University of Science and Technology, Tehran, ۱۶۸۴۶-۱۳۱۱۴, Iran
K Dashtian - Research Laboratory of Spectrometry & Micro and Nano Extraction, Department ofChemistry, Iran University of Science and Technology, Tehran, ۱۶۸۴۶-۱۳۱۱۴, Iran
R Zare Dorabei - Research Laboratory of Spectrometry & Micro and Nano Extraction, Department ofChemistry, Iran University of Science and Technology, Tehran, ۱۶۸۴۶-۱۳۱۱۴, Iran
M Mohammadian - Research Laboratory of Spectrometry & Micro and Nano Extraction, Department ofChemistry, Iran University of Science and Technology, Tehran, ۱۶۸۴۶-۱۳۱۱۴, Iran

Glutamic acid (Glu) is the most abundant excitatory neurotransmitter in the central nervous system, and an elevated level of Glu may indicate some neuropathological diseases. Herein, we developed a photoelectro-responsive fluorometric test strip-based peroxidase mimic activity of the triazine covalent organic framework/polyoxometalate heterojunction coated on paper for the effective luminol oxidation leading to a fluorogenic reaction and subsequently highly sensitive neuropathy biomarker glutamic acid detection. Results confirm the extensive π-electron delocalization, polarity, built-in electric field and high chemical stability for the as-prepared photo-electro-nanozyme. The increase in light harvesting and its separation and charge transfer were also confirmed by electrochemical and spectroscopic analysis. This photo-electronanonzymaticplatform revealed the sensitive detection of L-glutamic acid as a neurotransmitter in the linear range of ۵ μM to ۵۰۰ μM with a limit of detection (LOD) of ۱.۲ μM. The findings confirmed the safety and feasibility of the proposed photo-electro-nanonzymatic strategy for detecting L-Glu in biological samples as well as revealed satisfied selectivity in the presence of other biological molecules. This work opens a new insight into the development of signal-amplified test strips for point-of-care testing and disease monitoring.

Glutamic acid; neuropathy biomarker; photo-electro-responsive; fluorometric test strip; triazin covalent organic framework; polyoxometalate