عماد رضا - Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran
حسین عزیزی - Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran
علی اصغر احمدی - North Research Center, Pasteur Institute of Iran, Amol, Iran

Introduction: One of the vital enzymes during spermatogenesis, which is one of the pluripotency factors of stem cells and contributes to maintaining their pluripotency is alkaline phosphatase. ZBTB۱۶ and ZFP proteins are critical elements in stem cells which are expressed in pluripotent stem cells and maintain their pluripotency due to their role in messaging pathways. Material & Methods: The separation of the spermatogonial stem cells is the initial stage, followed by the preparation of the mouse embryonic stem cells and ES-like cells. The protein-protein network and the connections between genes were then investigated after alkaline phosphatase staining. Then, using the Fluidigm PCR method, the expression of the ZBTB۱۶ and ZFP genes was determined. Finally, spermatogonia was immunohistochemically stained for the ZBTB۱۶ protein. (Ethic Code: IR.AUSMT.REC.۱۴۰۰.۲۹) Findings: Alkaline phosphatase was expressed positively by stem cell types; moreover, it was absent in Sertoli and fibroblast cells. Additionally, the specificity of these factors was demonstrated by the positive expression of the ZBTB۱۶ and ZFP genes in these three cells and their negative expression in Sertoli cells. The connection and effect of these genes on the other genes are shown by the Protein-Protein network analysis. Discussion & Conclusion: Alkaline phosphatase, as well as ZBTB۱۶ and ZFP genes are expected to be among the special markers of pluripotent stem cells and the critical elements of embryonic stem-like cells.

Alkaline phosphatase, Embryonic stem cells, Spermatogonial stem cells (SSCs), ZBTB۱۶ protein, ZFP protein, سلول های بنیادی اسپرم ساز (SSC), سلول های بنیادی جنینی, آلکالین فسفاتاز, پروتئین ZBTB۱۶, پروتئین ZFP