Mahroo Seyed Jafari Olia - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran
Mehrdad Azin - Department of Biotechnology Iranian Reasearch Organization for Science and Technology, Tehran, P. O. Box ۳۳۵۳-۵۱۱۱, Iran.

In the wake of extensive fossil fuel use and CO۲ accumulation in the environment, biofuel production from microalgae may be more effective and leave a less environmental footprint. The nutritional and environmental factors and their interactions affect the growth performance and biochemical constitution of different microalgae. The behavior of microalgal cells in different culture scales depends on the mentioned factors. The present study evaluates the potential of two microalgae isolates, Picochlorum D۸, and Chlorella S۴, in different culture scales. High biomass and carbohydrate productivity were considered as important factors to identify the potent microalga. Acid-thermal pretreatment was applied to measure the carbohydrate concentration. The carbohydrate composition of selected microalga was investigated using thin-layer chromatography (TLC). According to the observations, Chlorella S۴ exhibited the best dry biomass and carbohydrate productivity of ۶۲ ± ۶ mg/L/d and ۱۹.۱۶ ± ۱.۵۷ mg/L/d in a ۲۰۰ L indoor open raceway pond, respectively. For Picochlorum D۸, the highest biomass productivity of ۲۶.۲۴ ± ۰.۶۲۵ mg/L/d and carbohydrate productivity of ۷.۴۵ ± ۰.۵۳ mg/L/d were achieved in a ۲ L Erlenmeyer flask. Based on TLC analysis, glucose, galactose, and xylose were detected as the main monosaccharides in Chlorella S۴ hydrolysate. The current study demonstrated Chlorella S۴’s capacity to produce biomass in a large-scale system. The relatively high carbohydrate content of this microalga makes it a promising raw material for potentially producing bioethanol.

Bioethanol, Carbohydrate content, Large-scale system, Microalgal biomass, Pretreatment, Thin-layer chromatography