Parastoo Azadbeigie - PhD in Cytogenetics,Associate Professor,Dept of Biology,Faculty of Sciences,Ferdowsi University of Mashhad( FUM)Iran.
Hamid Gholamhosseinian - Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Nazanin Shadanpour - PhD in Cytogenetics,Associate Professor,Dept of Biology,Faculty of Sciences,Ferdowsi University of Mashhad( FUM)Iran.
Fatemeh B.Rassouli - Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
Farhang Haddad - Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

Crocin is the main biologically active carotenoid of saffron, which is generally derived from the dried stigma of Crocus sativus L. Crocin has considerable pharmaceutical activities including antioxidant, anti-inflammatory, hepatoprotective, hypotensive, antidiabetic and anticancer effects. Gastric cancer is the fifth most common malignancy and the fourth leading cause of cancer-associated death worldwide. The aim of current study was to investigate toxic effects of crocin, alone and in combination with radiation, on human gastric cancer cells for the first time. To do so, MKN-۴۵ cells were treated with ۲ mM crocin for ۷۲ h. Meanwhile, cells were also treated with ۲ mM of crocin for ۲۴ h and then exposed to ۶۰۰ cGy X-ray followed by ۴۸ h recovery. Cell viability was determined after both approaches by alamarBlue assay. Results revealed that ۲ mM crocin decreased cell density and ۲ mM crocin + ۶۰۰ cGy X-ray decreased cell density and changed the morphology of MKN-۴۵ cells. Calculating the viability of cells also indicated that ۶۴% of cells were alive after ۷ h treatment with ۲ mM crocin, while this amount was decreased down to ۴۳.۲% upon combination of ۲ mM crocin + ۶۰۰ cGy X-ray treatment. Further investigation on other human gastric adenocarcinoma cell lines is recommended to better evaluate combinatorial effects of crocin and radiation in vitro.

Crocin, Radiation, Gastric cancer, Viability assay, Combination