David Shayan - Research Institute Molecular Biological System Transfer (MBST), Tehran, Iran, and ۲- Department of Cellular and Molecular Biology, Faculty of Biology, Kish International Campus, University of Tehran, Tehran, Iran
Brigitte Eckert - Research Institute Molecular Biological System Transfer (MBST), Tehran, Iran
Mohammad Habibi - National Reference Laboratory, Diagnosis and Applied Studies Center, Iran Veterinary Organization, Tehran, Iran
Navid Reza Shayan - Research Institute Molecular Biological System Transfer (MBST), Tehran, Iran
Shadab Esmaeilz - National Reference Laboratory, Diagnosis and Applied Studies Center, Iran Veterinary Organization, Tehran, Iran
Ali Akbar Afzalkhan - National Reference Laboratory, Diagnosis and Applied Studies Center, Iran Veterinary Organization, Tehran, Iran
Narges Amininia - Research Center Ticks and Tick-borne Diseases, Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran
Parviz Shayan - Research Institute Molecular Biological System Transfer (MBST), Tehran, Iran, and ۴- Research Center Ticks and Tick-borne Diseases, Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Iran

There are numerous RNA virus infections in mammals as emerging zoonosis originating from wildlife. The total viral diversity is unknown. The total number of mammalian viruses is estimated to be more than ۳۲۰۰۰۰. Many of these viruses have genomic RNA and are important as zoonotic agents. In many RNA virus infections, the virus load in the serum can be used as a marker for severity. For diagnostic purposes dealing with the low virus titer in serum and biotechnological applications, it is mandatory and highly important, to have a native, innovative, and sensitive RNA isolation kit. For this aim, we used an RNA isolation kit with RNA carrier, produced by Research Institute Molecular Biological System Transfer (MBST, Tehran, Iran) and the Avian infectious bronchitis virus vaccine of Nobilis, ۲۵۰۰ Doses, ۱۰۳ID۵۰ (Intervet, Netherlands) as virus RNA probe. The RNA was extracted from diluted solutions (up to ۱۰-۹). Subsequently, cDNA was synthesized. The cDNA was then amplified using a specific primer pair derived from the RNA genome of the Avian infectious bronchitis virus. Our results showed that it was possible to detect RNA viruses in the prepared samples with virus titers of up to ۱۰-۹ or ۰.۰۰۰۰۱ × ID۵۰. These results were confirmed by the Iranian National Reference Laboratory, Diagnosis and Applied Studies Center, and Veterinary Organization using their evaluation matrix Avian influenza virus sample. In conclusion, this kit is suitable for samples with low RNA virus titers.

RNA virus, RT-PCR, Carrier RNA, Avian infectious bronchitis virus, Avian influenza virus