P. Rahnemoon - Department of Food Biotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Islamic Republic of Iran.
M. Sarabi Jamab - Department of Food Biotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Islamic Republic of Iran.
M. Javanmard Dakheli - Department of Food Technology, Iranian Research Organization for Science and Technology ‎‎(IROST), Tehran, Islamic Republic of Iran.
A. Bostan - Department of Nanotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Islamic Republic of Iran.
O. Safari - Department of Fishery, Faculty of Natural Resources and Environment, Ferdowsi University of Mashhad, Mashhad, Islamic Republic of Iran.

The present study aimed to evaluate effectiveness of Superheated Solvent Extraction (SSE) compared with instant Controlled Pressure Drop (DIC) assisted Solvent Extraction (DIC-SE) on total phenolic, flavonoids, and anthocyanins compounds from pomegranate peels. The effects of temperature, extraction time, and water:ethanol ratio for SSE method, and temperature and heating time for DIC-SE were studied. The highest phenolic compounds, flavonoids, and extraction yields by SSE was achieved at ۱۶۰˚C, ethanol: water ۵۰:۵۰ and ۲۰ minutes, subsequently in the DIC-SE, the most effectiveness was approached at ۱۵۰˚C for ۵ seconds (P< ۰.۰۵). The SSE improved the total phenolic compounds (۵۶۳.۱۶±۱.۰۴ mg g-۱), anthocyanins (۲۸۵.۱۱±۱.۰۲ mg ۱۰۰ g-۱), extraction yield (۶۸.۷%) and shortened the extraction times compared to DIC-SE, but flavonoid content was more in DIC-SE extract (۴۳۹.۰۷±۰.۰۵ mg g-۱). Based on HPLC analyses, gallic acid was not detected in any of the obtained extracts, but the amount of ellagic acid and punicalagin A and B in DIC-SE extract was higher than SSE. The current study clearly shows that the SSE is an effective extraction method to obtain phenolic compounds and the DIC is an advantageous pretreatment for extraction of flavonoids from pomegranate peels.

Anthocyanins, Bioactive compounds, DIC, Flavonoids, SSE., پوست انار, حلال فوق داغ, پیش تیمار DIC, ترکیبات فنولی