Elimination of Grapevine Virus A (GVA) by Cryotherapy and Electrotherapy
عنوان مقاله: Elimination of Grapevine Virus A (GVA) by Cryotherapy and
Electrotherapy
شناسه ملی مقاله: JR_JASTMO-13-3_013
منتشر شده در در سال 1390
شناسه ملی مقاله: JR_JASTMO-13-3_013
منتشر شده در در سال 1390
مشخصات نویسندگان مقاله:
Sh. Bayati - Department of Plant pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
M. Shams-bakhsh - Department of Plant Protection, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
A. Moini - Department of Plant pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
خلاصه مقاله:
Sh. Bayati - Department of Plant pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
M. Shams-bakhsh - Department of Plant Protection, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
A. Moini - Department of Plant pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
The incidence of grapevine virus A (GVA) is reported from almost all of the major
grapevine growing regions in Iran. Grapevine is vegetatively propagated by rooting of
cuttings or grafting. In such plants, viral diseases are transmitted from stock plants to the
progeny. Therefore, the control of grapevine viruses can be achieved primarily through
production of healthy stock plants. In the present research, cryotherapy and
electrotherapy were employed for elimination of GVA from naturally infected vine (Vitis
vinifera L. cv Black) and their efficiency was compared. In cryotherapy, ۵۹% of the shoot
tips survived and regenerated into whole plants, of which ۴۲% were free of GVA detected
by RT-PCR. In the electrotherapy, the effects of electric current value and treatment
duration were investigated on plant survival and virus elimination. The best results were
obtained by using ۳۰ milliamps (mA) for ۱۵ minutes. With this treatment, survival and
virus-free frequencies were about ۶۲% and ۴۰%, respectively. This is the first report of
electrotherapy of grapevine shoot tips as a potential tool for GVA elimination. The results
showed that cryotherapy was a more efficient and convenient protocol than
electrotherapy for elimination of GVA from infected grapevine.
کلمات کلیدی: RT-PCR, Cryotherapy, Electrotherapy, Shoot tips, Vitis
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1827281/