Ali Jafari-Mofidabadi - Golestan Research Center of Agriculture and Natural Resources, P.O Box ۴۹۱۵۶۷۷۵۵۵ Gorgan, Iran
Iman Jafari - Seed & Plant Certification and Registration Institute, Karaj, Iran
Tayebe Shomali - Mazandran University of Medical Sciences, Faculty of Pharmacy, Sari, Iran

For sexual reproduction of Allium hirtifolium, collected bulbs were planted in the field. In order to develop ovary embryo and seed setting, control self-pollination and natural open pollination took placed using honey bees under cages. Ovary development and seed setting of A. hirtifolium extraordinary were affected by pollination and there was a significant difference between mean effect of self-pollination and natural open pollination using chi-Square test at α=۰.۰۵%. Open pollination using honey bees with average ۲۳ numbers of seeds per flower stalk showed higher seed formation than control self- pollination. Out of ۲۴۰ mature ovaries (۲۵ and ۳۵ day-old ovary), which have been isolated, ۱۹۴ ovary were germinated (۸۰.۸%) from ۲۵ and ۳۵ days after pollination (DAP). Due to long embryo development in this species, all isolated embryo (less than ۲۵ day-old) failed to develop direct plantlets on either MS hormone free or half-MS medium, for in vitro germination. Analysis of data originated from affect of culture media on embryo germination, indicated that there is no significant differences between culture media on embryo germination using Chi-square test at α=۰.۰۵ level. In spite of no differences, MS media showed higher ovary germination (average ۵۴.۳۳%) than half-MS medium (average ۴۱.۵۰%). Successful acclimatized plantlets transferred to green-house and then to the field.

Allium hirtifolium, Mooseer, OVARY, Embryo, sexual reproduction, pollination