Soraya Sajadimajd - Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
Faranak Aghaz - Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Elham Arkan - Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Gholamreza Bahrami - Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

Objective(s): Skin wounds are appraised as a rapidly growing threat to the economy and public health. Wound management is the main goal to promote rapid repair, with functional and esthetic outcomes. Among several wound healers, ointments are the most cost-effective and highly functional.  Materials and Methods: Here, polysaccharide was isolated from Rosa canina and structural analysis was performed by NMR, LC-MS/MS, and FTIR. Then, the polysaccharide was encapsulated in SLN through the dialysis process. Structural analysis was committed to a survey on the physical and chemical properties of polysaccharide-SLN (PS-SLN) complex by Uv-Vis spectrophotometry, dynamic light scattering (DLS), and scanning electron microscopy (SEM) technologies. The ointment was prepared by adding PS-SLN to R. canina oil and beeswax.  Results: The prepared PS-SLN nanoparticles had monodispersity with a size of about ۲۱۷ nanometers.  The nano-ointment showed high stability with a pH of about ۶ and a high density near ۳۲۵۶ centipoises. The skin absorption of the compounds was determined by the Franz cells. The in vitro skin absorption indicated that during the first ۱۲ hr ۳۶% of our nano-ointment’s skin permeation and then continued up to ۱۲ hr to ۵۱%. The higher healing rate of nano-ointment than positive control with no allergic effects confirmed its efficiency in wound management.  Conclusion: The results indicated that the nano-ointment could be applied for the healing of scars in pre-clinical and clinical trials. Owing to effectual scar healing, nano-ointment may be effective in the treatment of other wounds including burn, diabetic and chronic ones.

Encapsulation, Nano-ointment, Polysaccharide, SLN, Wound healing