Sarah Mamdouh Shoeib - Clinical pathology department, Faculty of Medicine, Tanta University, Egypt.
Doaa Elwy Abdeldaim - Public Health Department, Faculty of Medicine, Tanta University, Tanta.
Shimaa Samir Mashal - Internal Medicine department, Faculty of Medicine, Tanta University, Egypt.
Rowida Raafat Ibrahim - Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Tanta University, Egypt.
Lamees Mohamed Dawood - Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Tanta University, Egypt.
Doaa Shatat - Gynecology and Obstetrics Department, Faculty of Medicine, Tanta University, Egypt.
Yasmine Ibrahim El-Masry - Gynecology and Obstetrics Department, Faculty of Medicine, Tanta University, Egypt.
Ahmed Almeldin - Physiology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Radwa Mahamoud El Sharaby

Background: Preeclampsia (PE) is a multisystem pregnancy disorder that increases maternal-perinatal morbidity and mortality significantly. MicroRNA-۱۵۵ (miR-۱۵۵) overexpression in the sera of pregnant women has been linked to preeclampsia. Researchers discovered that miR-۱۵۵ acts during pregnancy by down-regulating and reducing the cysteine-rich angiogenic inducer ۶۱ (CYR۶۱), which causes local ischemia as well as oxidative stress. Methods: The level of miR-۱۵۵ expression in all serum samples was quantified using real-time polymerase chain reaction (RT-PCR), and serum CYR۶۱ was measured using enzyme-linked immunosorbent assays. Together with the Cyr-۶۱/miR-۱۵۵ ratio, they were evaluated as biomarkers for PE pathogenesis and severity prediction. Results: MiR-۱۵۵ expression, serum CYR۶۱ levels, and Cyr-۶۱/miR-۱۵۵ ratios were all significantly higher in PE patients compared to the control group. Serum CYR۶۱ levels and the Cyr-۶۱/miR-۱۵۵ ratio differed significantly between mild and severe PE patients. Conclusion: MiR-۱۵۵ expression, serum CYR۶۱ levels, and Cyr-۶۱/miR-۱۵۵ may serve as biomarkers for PE pathogenesis and severity prediction.

Biomarker, Cysteine-Rich Protein ۶۱, MicroRNAs, Pre-Eclampsia.