Jamileh Sadat Mirsanei - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mahsa Nazari - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Ronak Shabani - Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran.
Azam Govahi - Endometriosis Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Sahar Eghbali - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Marziyeh Ajdary - Endometriosis Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Rana Mehdizadeh - School of Dentistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Atieh Sadat Mousavi - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mehdi Mehdizadeh - Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran.

Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an ۸۰% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U۲۵۱ cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells’ viability and apoptosis. Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for ۲۴, ۴۸, and ۷۲ hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of ۷۵ µg/ml for ۴۸ hours according to the MTT results. After ۴۸ hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-۲, and CASPASE-۳). Results: According to our results, the rod-shaped silver nanostructures had a size of about ۵۰ nm, increased apoptotic markers, including BAX and CASPASE-۳, and induced cell death. Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia. Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an ۸۰% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U۲۵۱ cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells’ viability and apoptosis. Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for ۲۴, ۴۸, and ۷۲ hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of ۷۵ µg/ml for ۴۸ hours according to the MTT results. After ۴۸ hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-۲, and CASPASE-۳). Results: According to our results, the rod-shaped silver nanostructures had a size of about ۵۰ nm, increased apoptotic markers, including BAX and CASPASE-۳, and induced cell death. Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia.

Apoptosis, Cell viability, Nanostructures, T-cell acute lymphoblastic leukemia.