Fatemeh Bazari Jamkhaneh - Sana Institute of Higher Education, Sari, Iran
Mina Owrang - Faculty of Medicine, Sari branch, Islamic Azad University, Sari, Iran
Hamid Reza Goli - Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Background: Increased resistance to β-lactams is one of the most important considerations about Pseudomonas aeruginosa. This study aimed to investigate the prevalence of blaOXA-۲ and blaOXA-۱۰ genes in β-lactam-resistant P. aeruginosa. Methods: In this study, ۱۰۰ clinical isolates were collected, and β-lactam-resistant strains were identified using the disk agar diffusion test. Bacterial DNA was extracted by the sodium dodecyl sulfate method, and the polymerase chain reaction was used to identify blaOXA-۲ and blaOXA-۱۰ β-lactamase genes. Results: In total, ۴۳ isolates were resistant to at least one of the β-lactams tested. Piperacillin-tazobactam and aztreonam were the most and least effective antibiotics, respectively. The resistance rate to a panel of β-lactams ranged from ۱۲% to ۳۷%. The highest rate of antibiotic resistance was related to wound and catheter specimens in burn and intensive care units. A significant relationship was observed between β-lactams resistance and the presence of blaOXA-۲ and blaOXA-۱۰ genes. Among ۴۳ resistant isolates, ۱۰۰% and ۸۳.۷۲% carried the blaOXA-۲ and blaOXA-۱۰ genes, respectively. Conclusion: High β-lactam resistance rates and prevalence of blaOXA-۲ and blaOXA-۱۰ in this study indicate these enzymes' significant role in resistance to β-lactams, possibly due to overuse of these antibiotics and their easier access.

Pseudomonas aeruginosa, blaOXA-۲, blaOXA-۱۰, beta-lactam resistance