Masumeh Rezaei - Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Arman Esmailzadeh - Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Ahmad Shanei - Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Background: In the bystander effect, non-irradiated cells receive biological signals from adjacent irradiated cells and undergo a variety of alterations, considered recently in non-ionizing irradiation like ultrasound waves. In this study, the bystander effect of therapeutic ultrasound exposure alone and in combination with cisplatin was determined.Objective: This study aims to determine the bystander effect caused by ultrasound and cisplatin.Material and Methods: This experimental study was conducted on the human melanoma cell line including two groups of target and bystander cells. The target cell group was divided into three sub-groups of ultrasound irradiation alone, cisplatin alone, and ultrasound irradiation in the presence of cisplatin that the culture medium of these three groups of cells was transferred to the bystander cell group using the medium transfer technique. Then, apoptotic bystander cells and the expression of P۵۳ and HO-۱ in target and bystander groups were measured.Results: The results of the MTT (۳-(۴,۵-dimethylthiazol-۲-yl)-۲,۵-diphenyltetrazolium bromide) and apoptosis assay showed that cell death in target and bystander groups receiving the ultrasound with cisplatin is higher than in the ultrasound without cisplatin. PCR (the polymerase chain reaction) results in the target and bystander groups receiving treatments with increased expression of the P۵۳ gene. Target and bystander groups receiving the ultrasound without cisplatin showed a decrease in HO-۱ gene expression, while the ultrasound with cisplatin showed an increase in the HO-۱ gene compared to the control group. Conclusion: Combining ultrasound with ultrasound and without it can transfer bystander signals to the cells that are not directly treated.

Bystander effect, Ultrasonic Therapy, Cisplatin, Melanoma, apoptosis