علیرضا مولازاده - Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran
راضیه رنجبر - Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran.
امین دخیلی اردستانی - Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran
کامران رنجبر - Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran
سید امین کوهپایه - Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran
مریم مقبول - Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran
سهراب نجفی پور - Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran
فاطمه نجفی پور - Ghutbuddin Clinic, Shiraz University of Medical Sciences, Shiraz, Iran

Background & Objectives: Breast cancer, a significant threat to women's health, is associated with a high annual mortality rate. Oxidative stress, characterized by the accumulation of reactive oxygen species, plays a pivotal role in cancer development. Ferula assafoetida, a traditional medicinal plant, has been widely used in cancer treatment. This study aims to compare the cytotoxic effects, phytochemical profiles, and antioxidant activities of Ferula assafoetida gum (FAGE) and leaf (FALE) extracts. Materials & Methods: The herbal extracts from FAGE and FALE were prepared using the maceration method in ۷۰% v/v ethanol. The phenolic content and radical scavenging activity of the extracts were assessed using the Folin-Ciocalteu and diphenylpicrylhydrazyl (DPPH) assays, respectively. The cytotoxic effects of the extracts were examined on the MDA-MB-۲۳۱ and MCF-۷ cell lines through the MTT assay. Additionally, the morphological changes of the cell lines were observed using an inverted microscope. Results: The phenolic content in FAGE (۱۴۲.۲۵± ۱۹.۳۵ µgGAE/mg) was significantly higher (P=۰.۰۱) than that in FALE (۴۸.۱۵± ۳.۰۱ µgGAE/mg). Additionally, the radical scavenging activity and cytotoxic effects of FAGE were also significantly stronger than those of FALE (P< ۰.۰۵). Regarding the cytotoxic effects of the extracts, FAGE exhibited IC۵۰ values of ۸۰.۵۹± ۱۰.۹۸ and ۵۹.۳۹± ۱۵.۸۹ µg/mL for MCF-۷ and MDA-MB-۲۳۱ cells, respectively, after ۷۲ hours of incubation. Conclusions: FAGE exhibited a greater cytotoxic effect on the MDA-MB-۲۳۱ cell line than on the MCF-۷, possibly following an estrogen-independent pattern. It appears that FAGE holds significant potential in refining oxidative stress, thereby inhibiting cell proliferation.

Phenol, Flavonoid, Oxidative stress, Cytotoxicity, Cancer