Enayatollah Asadmanesh - Department of Physical Education and Sport Sciences, Shiraz University, Shiraz, Iran
Maryam Koushki Jahromi - Department of Physical Education and Sport Sciences, Shiraz University, Shiraz, Iran
Farhad Daryanoosh - Department of Physical Education and Sport Sciences, Shiraz University, Shiraz, Iran
Javad Neamati - Department of Physical Education and Sport Sciences, Shiraz University, Shiraz, Iran
Mahdi Samadi - Department of Physical Education and Sport Sciences, Shiraz University, Shiraz, Iran

Introduction: Although resistance training and resveratrol is recommended as a stimulant for muscle regeneration in healthy subjects their effects on cancer-related cachexia indices are unclear. This study aimed to investigate the effect of a course of resistance training with resveratrol supplement on PAX-۷ and eMHC in Gastrocnemius muscle tissue of mice bearing colon cancer induced cachexia. Methods: This was an experimental study conducted on ۲۰ six-week-old BALB/c mice implanted with CT-۲۶ tumor. The mice were randomly divided into four groups: resistance training, resveratrol, resveratrol with resistance training, and control. The resistance training group performed ۶ weeks of progressive resistance training, the resveratrol group received ۱۰۰ mg/kg resveratrol per day. ۴۸ hours after the last experimental session, the mice were sacrificed and PAX-۷ and eMHC protein levels were measured using ELISA test through specimen taken from gastrocnemius muscles. Statistical test of one way ANOVA was used for data analysis. Results: PAX-۷ level was significantly lower in resveratrol with resistance training group compared to control (P=۰.۰۰۹), resveratrol (P=۰.۰۰۵), and resistance training groups (P=۰.۰۰۲). eMHC was significantly lower in the resistance training group compared to the control (P=۰.۰۸), resistance training, and resveratrol groups (P=۰.۰۰۲). Conclusion: Resistance training was not an appropriate intervention for treating reduced muscular regeneration due to cachexia. However, regarding reducing effect of resveratrol supplementation with resistance training on PAX-۷ level, it may be recommended to improve muscular regeneration.

Resistance Training, Resveratrol, PAX-۷, eMHC, Cancer, Cachexia