Ali Bahari - Department of Physics, University of Mazandaran, Babolsar, Mazandaran, Iran.
Salman Ahmady-Asbchin - Department of Microbiology, University of Mazandaran, Babolsar, Mazandaran, Iran.
Masoumeh Naeij - Department of Physics, University of Mazandaran, Babolsar, Mazandaran, Iran.
Abbas Farhadikoutenaei - Department of Physics, University of Mazandaran, Babolsar, Mazandaran, Iran.
Ali Al-Jilef - Department of Physics, University of Mazandaran, Babolsar, Mazandaran, Iran.

Recently, the synthesis of metal nanocrystallites using plant extracts has received much attention due to its low cost and compatibility with the environment. In this research, copper nanocrystallites were synthesized using hawthorn extract. The formation of copper nanocrystallites was observed by changing the color of the solution. Synthesized copper nanocrystallites were investigated by techniques such as: Ultraviolet-Visible Spectroscopy (UV-Vis), Scanning Electron Microscope (SEM), Fourier Transform-Infrared Spectroscopy (FTIR), Energy Dispersion Spectroscopy (EDX) and X-ray Diffraction Spectroscopy (XRD). In the UV-Vis spectrum, a dominant absorption peak at ۵۵۲ nm wavelength was observed, which was related to copper nanocrystallites. In the images taken from SEM, it was found that the nanocrystallites are nearly spherical in shape and their average size on the surface of the sample was about ۶۱-۸۵ nm.  Parallerly, in the investigation of antibacterial properties, synthesized nanocrystallites showed better antibacterial activity against Gram-negative bacteria (Escherichia coli) respect to other bacteria in the present work. The obtained results show that hawthorn extract can be used for the synthesis of copper nanocrystallites.

Antibacterial, Copper Nanocrystallites, Green synthesis, plant extract, X-ray Diffraction Spectroscopy (XRD)