Fatemeh Barani - Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
Mohammad Ali Haghighi - Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran The Persian Gulf Tropical Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, Iran
Mehrandokht Sirous - Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Behrouz Naeimi - Department of Medical Laboratory Sciences, Faculty of Paramedical, Bushehr University of Medical Sciences, Bushehr, Iran
Bahram Ahmadi - Department of Medical Laboratory Sciences, Faculty of Paramedical, Bushehr University of Medical Sciences, Bushehr, Iran

BACKGROUND AND OBJECTIVESCholera is an acute diarrheal infection with epidemic potentials, caused by Vibrio cholerae. In the recent years, V. cholerae strains have emerged as a multidrug resistance of enteropathogenic bacteria. The antibiotics could be ineffective against infections causing multidrug resistant enteric pathogenic bacteria and phytodrug could be helpful for the control of these infections. Syzygium cumini is a tropicl medicinal fruit show various therapeutic applications as antibacterial and antidiarrheal effects. The aim of current study was to investigate the effect of S. cumini leaf extract against Non-O۱/Non-O۱۳۹ V. cholerae clinical isolates.MATERIALS AND METHODSThe antibacterial activity of aqueous and ethanolic leaf extracts of S. cumini were evaluated against Non-O۱/Non-O۱۳۹ V. cholerae clinical isolates. Three clinical isolates (completely susceptible, antibiotic resistant, MDR) of V. cholerae were analyzed for assessment of antivibriocidal activity of S. cumini extracts. Minimum inhibitory concentration (MIC) of extracts was determined by the micro-broth dilution method.RESULTS AND DISCUSSION The aqueous & ethanolic leaf extracts of S. cumini were able to inhibit the growth of Non-O۱/Non-O۱۳۹ V. cholerae clinical isolates with a noteworthy MIC value of ۷۵۰۰ and ۲۵۰۰ μg/ml for susceptible strain, ۵۰۰ and ۲۰۰۰ μg/ml for antibiotic resistant strain and ۱۰۰۰ and ۲۰۰۰ μg/ml for MDR strain, respectively. In this study, leaf extracts of S. cumini was exhibited potential activity against all susceptible, antibiotic resistant and MDR isolates of V. cholerae.CONCLUSION Now a days, increased antimicrobial resistance is the cause of severe infections and increased mortality, thus safer novel antibacterial agents are required. In present study, the leaf extracts of S. cumini showed antivibriocidal effects especially against MDR clinical isolate. This outcome is remarkable considering that leaf extract of S. cumini seem to be promising as a complementary medicine to control of cholera infection, because of its potential for inhibiting MDR V. cholerae and its less cytotoxic properties

Vibrio cholerae, Syzygium cumini, Multidrug resistance