Zahra Sabouri - Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Shirin Sammak - Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Sajjad Sabouri - Natural Disasters Prevention Research Center, School of Civil Engineering, Iran University of Science and Technology, Tehran, Iran
Samaneh Sadat Tabrizi Hafez Moghaddas - Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Majid Darroudi - Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran

This study was conducted to evaluate the photocatalytic applications and the cytotoxicity effects of Ag-Se doped ZnO-Co۳O۴-NiO fivenary nanocomposite synthesized using polyanionic cellulose (PAC) polymer as a stabilizer agent. Several procedures such as XRD, FESEM, FTIR, EDX, PSA, and UV-Vis were applied to investigate synthesized nanocomposite. Consistent with the FTIR spectrum, chemical bonds were seen in the structure of the nanocomposite which approved the successful synthesis of them. The XRD pattern of the synthesized nanocomposite revealed sharp diffraction peaks with high crystallinity, and pure phases of Se, Ag, ZnO, NiO, and Co۳O۴ were approved with XRD analysis. FESEM/PSA images indicate that nanocomposite was synthesized with an average size of ۲۳-۴۹ nm and relatively uniformly distributed, in addition, it has a spherical morphology. The synthesized nanocomposite exhibited excellent photocatalytic activity to methyl orange (MO) dye degradation under a UVA light source. The degradation rate of nanocomposite reached ۹۹% within ۸۰ min. The kinetic studies indicate that the degradation of MO dye follows a first-order kinetic model. The cytotoxicity of the nanomaterial was assessed on normal mouse fibroblast NIH۳T۳ and cancer mouse melanoma B۱۶F۰ cell lines with the MTT assay. The results of the MTT test revealed significant cytotoxic influences on cancer B۱۶F۰ cells (IC۵۰ value = ۲۵۸.۵ µg/mL) in comparison to normal cells.

Ag-Se doped ZnO-Co۳O۴-NiO fivenary Nanocomposite, Poly Anionic Cellulose, Photocatalytic, Anticancer