Alireza Rafighdoust - Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Nasser Mahdavi Shahri - Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
Javad Baharara - Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Objective(s): Use of biological scaffolds and automating the cells directing process with materials such as growth factors and glycosaminoglycans (GAGs) in a certain path may have beneficial effects in tissue engineering and regenerative medicine in future. In this research, chondroitin sulfate sodium was used for impregnation of the scaffolds. It is a critical component in extracellular matrix and plays an important role in signaling pathway; however, little is known about its role within mammalian development and cell linage specification. Materials and Methods: Due to its porous and appropriate structure and for putting cells in ۳D space, the kidney of BALB/c mouse was selected and decellulalized using physical and chemical methods. After decellularization, the scaffold was impregnated in chondroitin sulfate solution (CS) for ۲۴ hr. Then, ۶۰×۱۰ ۵ human adipose-derived mesenchymal stem cells were seeded on the scaffold to assess their behavior on day ۵, ۱۰, ۱۵, ۲۰, and ۲۵. Result: After ۴۸ hr, DAPI staining approved completed decellularized kidney by ۱% SDS (sodium dodecyl sulfate). Migration and establishment of a number of cells to the remaining area of the glomerulus was observed. In addition, cell accumulation on the scaffold surface as well as cells migration to the depth of kidney formed an epithelium-like structure. Up to the day ۱۵, microscopic study of different days of seeding showed the gradual adhesion of large number of cells to the scaffold. Conclusion: Glycosaminoglycan could be a right option for impregnation. It is used for smartification and strengthening of natural scaffolds and induction of some behaviors in stem cells.

Acellular scaffold, Chondroitin sulfate, Mesenchymal stem cell, Cell communication, Cell interaction, Extracellular matrix