A Ghalyanchi Langroudi - Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran,Iran
H Hosseini - Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj,Iran
V Karimi - Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
O Madadgar - Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran,Iran

BACKGROUND: Newcastle disease virus (NDV) is the causative agent of the Newcastle disease (ND), a highly contagious disease in birds that causes significant economic losses to the poultry industry worldwide. ND is endemic in Iran and outbreaks are reported regularly in commercial poultry flocks and different species of birds. OBJECTIVES:The current study was carried out to characterize NDV based on phosphorprotein (P) gene from recent outbreaks in Iran, 2010-2012. METHODS: The P genefragment of NDV isolates of five chickens, 1 ostrich, and 1 Pigeon paramyxovirus-1 was obtained by RT-PCR and sequenced. RESULTS: Phylogenetic analysis of sequences revealed that chicken and ostrich NDV isolates were closely related and placed in the genotype VII and Pigeon Paramyxovirus-1 was located in the genotype V. CONCLUSIONS:This is the first report of Phosphoprotein gene sequences of NDV strains isolated in Iran. This study will help us to understand the epidemiology andmolecular characteristics of Newcastle disease virus in Iran

Newcastle disease, phosphoprotein,phylogenetic study