Fatemeh Atabaki pasdar - Department of Anatomical Sciences, Urmia University of Medical Sciences, Urmia, Iran
Alireza Khooei - Department of Pathology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
Alireza Fazel - Department of Anatomy and Cell Biology, Mashhad University of Medical Sciences, Mashhad, Iran.
Maryam Rastin - Immunology Research Center, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Background: Differential diagnosis between complete hydatidiform mole, partial hydatidiform mole and hydropic abortion, known as hydropic placentas is still a challenge for pathologists but it is very important for patient management. Objective: We analyzed the nuclear DNA content of various types of hydropic placentas by flowcytometry. Materials and Methods: DNA ploidy analysis was performed in 20 non-molar (hydropic and non-hydropic spontaneous abortions) and 20 molar (complete and partial moles), formalin-fixed, paraffin-embedded tissue samples by flow cytometry. The criteria for selection were based on the histopathologic diagnosis. Results: Of 10 cases histologically diagnosed as complete hydatiform mole, 9 cases yielded diploid histograms, and 1 case was tetraploid. Of 10 partial hydatidiform moles, 8 were triploid and 2 were diploid. All of 20 cases diagnosed as spontaneous abortions (hydropic and non-hydropic) yielded diploid histograms. Conclusion: These findings signify the importance of the combined use of conventional histology and ploidy analysis in the differential diagnosis of complete hydatidiform mole, partial hydatidiform mole and hydropic abortion

Flow cytometry, Hydatidiform mole, Spontaneous abortion, Ploidy