Mohsen Alipoor - Ph.D student, Department of Nanobiotechnology, Faculty of bioscience, University of TarbiatModares, Tehran , Iran
Saman Hosseinkhani - Professor, Department of Biochemistry, Faculty of bioscience, University of Tarbiat Modares, Tehran,Iran
Reza Sheikhnejad - Professor, Tofigh Daru Res. & Eng. Tehran, Iran
Asia Majidi - Ph.D student, Department of Nanobiotechnology, Faculty of bioscience, University of TarbiatModares, Tehran , Iran

Gene therapy is one of the promising approaches for breast cancer treatment. However, nucleic acid based drugs suffer from their poor delivery. Peptide-based nanocarriers have been shown to improve delivery of plasmid and SiRNA into living cells. To improve targeting gene delivery tobreast cancer cells, we have designed a peptide based nanocarrier, by combination of iRGD, as a targeting moiety, and MPG H1, as an effective gene delivery carriers. Materials and methods: In this study, the coding sequence of this peptide was synthesized by solid phase synthesis.After that, this fragment was ligated in pET-28a Plasmid and transformed into E. coli BL21. Finally, the purified peptide was characterized by Dynamic light scattering (DLS) analysis and gel retardation assay in agarose gel. Result: The result of DLS showed that the purified nanocarrier has a mono disperse size distribution with a mean hydrodynamic diameter of 30 nm. Gel retardation assay revealed that this peptide successfully condensed plasmid included luciferase gene. Discussion & conclusion: The physicochemical characteristics of nanocarrier were in principle, wellsuitedfor cell-targeted, in vivo administered nucleic acid –based drug. Furthermore the presence of an iRGD motif in our designed nanoparticle will be ensured specific gene delivery to breast cancer cells

Breast cancer, Gene therapy, Nanocarrier, Peptide