Mohammad Jalal Afsharmanesh - Master of Science Microbiology,
Mehrdad Shamsaddini - Scientific Member of Biological Manufactures of Research and Production Department, Kerman Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran

Clostridium perfringens(C. perfringens) is a Gram-positive, rod-shaped, spore-forming, and anaerobic bacteria that cause illness and death in humans and animals . Four main groups of C. perfringens toxins include alpha, beta, epsilon and iota, and accordingly classified into five types A to E, that type A produced alpha toxin exclusively. At first The main subject of this research was mortality rate of toxin alpha secreted from C. perfringens type A. C. perfringens type A, cultured on basis enrichment medium in anaerobic conditions and incubated for overnight. test. From each dilution, 0.5 Then, the dilutions of suspension was prepared for MLD(Minimum Lethal Dose) ml intravenously injected into mice, and the results were evaluated after 72 hours. The minimum mortality rate of alpha toxin obtained 40.

Clostridium perfringens, Toxin, Culture, MLD