Mahsa Poorjangi - Medical student, students research committee , Zahedan University of Medical Sciences , Zahedan, I.R.Iran
Mohammad Bokaeian - Infectious Disease and Tropical Medicine Research Center, Zahedan University of Medical Sciences,Zahedan, IR Iran
Masume Farsizaban - Medical physics, Zahedan University of Medical Sciences, Zahedan, I.R.Ira

Citrullus colocynthis Schrad. shows antibsacterial and anticandidal properties. The folk medicinal use as a broad-spectrum antimicrobial agent is validated. The present study was carried out to determine the evaluation of antibacterial activity on Citrullus colocynthis seed extract. In this study, the antibacterial activity using 9 Gram-positive and Gram-negative bacterial strains Streptococcus pyogenes ATCC® 19615, Streptococcus pneumoniae ATCC 49619, S. saprophyticus ATCC®15305, Hafnia alvei ATCC 51873, Acinetobacter baumannii ATCC 19606, Enterococcus faecalis ATCC 29212, Proteus mirabilis ATCC 35659, Serratiamarcescens ATCC 274 and Staphylococcus aureus ATCC® 25923 with methods of MIC, MBC were studied and resistance to standard antibiotics, erythromycin, cefixime, ceftazidime, tetracyclin, ampicillin and amikacin were compared. The result show that the highest inhibitory (MIC) for the C. colocynthis seed 100 ppm concentration which 3 strain was inhibited at this concentration. The highest fatality rate (MBC) for the C. colocynthis seed is 200ppm, which the bacteria Streptococcus pneumoniae and Proteus mirabilis has been lost. While the lowest fatality rate (MBC) for the C. colocynthis seed is 50ppm.

Citrullus colocynths’, ‘Antibacterial’, ‘ activity’, ‘ extract’