Sara Khaleghi - Medical Biology Research Center, Kermanshah University of Medical Sciences
Mitra Bakhtiari - Fertility & Infertility Research Center, Kermanshah University of Medical Sciences, Fertility & Infertility Research Center, Kermanshah University of Medical Sciences

Background: Semen cryopreservation produces significant amounts of reactive oxygen species (ROS), which may lead to impairment of sperm morphology, function, and ultimately, male fertility. Since Tribulus terrestris has antioxidant and free-radical-scavenging properties, this study aims to reveal the effect of the Tribulus terrestrisextract on motility and vitality of human sperms after cryopreservation. Methods: Semen specimens from 80 healthy volunteers were divided into eight groups: fresh control (group I), freeze control (group II), groups III, IV, and V, which had 20, 40, and 50 mg/mL doses of Tribulus terrestris extract added before cryopreservation, and groups VI, VII, and VIII, which were supplemented by these extract doses after the freeze-thaw process. To evaluate the effects of the Tribulus terrestris extract, the semensamples were incubated with the extract and evaluated with a light microscope for motility and viability. Results: After cryopreservation, a significant improvement in spermatozoa viability was observed in group VII. In groups VII and VIII, motility, according to World Health Organization (WHO) criteria, increased considerably (p <0.001). There was no significant difference among groups III, IV, and V. Conclusion: The present study demonstrated that the protective effects of Tribulus terrestris, which improves human sperm motility and viability, may be due to its antioxidant properties. On the basis of the results, the researchers concluded that Tribulus terrestris can be used as a safe therapeutic alternative to current modalities for the management of motility dysfunction in males.

Tribulus Terrestris, Human Sperm,Motility, Viability, Cryopreservation, Reactive Oxygen Species