Seyedeh Mina Amiri-Moghaddam - Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
Ahmad Reza Bahrami - Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology,Ferdowsi University of Mashhad, Mashhad, Iran
Ali Emadi Torghabeh - Cancer Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Ladan Goshayeshi - Department of Gastroenterology and Hepatology, Faculty of Medicine, Mashhad University of MedicalSciences, Mashhad, IranGastroenterology and Hepatology Research Center, Mashhad University of Medical Sciences,Mashhad, Iran

Introduction: Gastric cancer (GC) is classified into cardia and non-cardia types depending ontumor site. Various epigenetic and genetic variations are implicated in gastric carcinogenesis.CCAT2, long non coding RNA (lncRNA) in the 8q24 has been attributed to the progression ofmultiple human cancers. Its up regulation has been reported in gastric cancer, making it potentialbiomarker for GC diagnosis and prognosis. Accumulating evidence suggests the functionality oflncRNAs in cancer development and introduces their variations as genomic biomarkers fordetermining cancer predisposition and treatment outcomes. rs6983267 is single nucleotidepolymorphism within CCAT2 lncRNA that its association with GC has been reported in someethnic populations, although, its mechanism of action remain unclear. Therefore, present study hasbeen done to investigate the association of rs6983267 with different types and histological grades ofGC.Materials Methods: TaqMan genotyping assay was used to determine the genotypes of 68patient and 82 control DNA samples. GC samples composed of 38 non-cardia and 30 cardia types.Chi2 test was used to find association of this SNP with gastric cancer susceptibility. Stratifiedanalysis was also done for association in cardia and non-cardia and different grades of GC.Results: TaqMan genotyping assay was used to determine the genotypes of 68 patient and 82control DNA samples. GC samples composed of 38 non-cardia and 30 cardia types. Chi2 test wasused to find association of this SNP with gastric cancer susceptibility. Stratified analysis was alsodone for association in cardia and non-cardia and different grades of GC.Conclusion: Our results suggest that allele is more frequent in more aggressive tumors, pointingto its involvement in tumor invasion and metastasis, but it needs greater sample size to be able todraw conclusive conclusion